Identification of Potential Isolate Phosphate Solubilizing Bacteria Used 16S rRNA Gene

Plants always interact with microorganisms either negatively or positively during their life cycle. The Phosphate Solubilizing Bacteria (PSB) group is one example that has some beneficial effects on the plant. PSB can transform insoluble soil inorganic phosphates into soluble phosphorous so plants absorb them. This research aimed to test isolate's ability solubilize and use 16S rRNA marker gene identify it. Testing of bacteria Pikovskaya's agar medium used a qualitative method by observing halo zone in colony around Then bacterial isolate was identified analyzing result sequencing gene. RZ02 showed highest performance solubilizing phosphorous, 9.1 mm solubilization index. carried out using primers 27F 1492R an amplicon size 1500 base pairs. Sequencing analysis construction phylogeny tree were conducted Mega X neighbor-joining 100% similarity Pseudomonas aeruginosa OIS 481. index indicated had potential candidate be inoculant components for biofertilizer.